UNIT 1.11 Homozygosity Mapping Using Pooled DNA

  1. Val C. Sheffield

Published Online: 1 MAY 2001

DOI: 10.1002/0471142905.hg0111s13

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Sheffield, V. C. 2001. Homozygosity Mapping Using Pooled DNA. Current Protocols in Human Genetics. 13:1.11:1.11.1–1.11.21.

Author Information

  1. University of Iowa, Iowa City, Iowa

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAY 1997


Genomic screening is a common and popular method for localizing human disease genes. Genotyping each individual sample can be a laborintensive and expensive process. This unit describes the technique of pooling DNA from many related affected individuals, genotyping the resulting pools, and scoring the results. This technique can greatly reduce the effort required to screen for genetic linkage and is particularly useful when applied to inbred populations. The unit includes a table showing a pool of human STRP markers developed by the Cooperative Human Linkage Center.