UNIT 1.11 Homozygosity Mapping Using Pooled DNA
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Human Genetics
How to Cite
Sheffield, V. C. 2001. Homozygosity Mapping Using Pooled DNA. Current Protocols in Human Genetics. 13:1.11:1.11.1–1.11.21.
- Published Online: 1 MAY 2001
- Published Print: MAY 1997
Genomic screening is a common and popular method for localizing human disease genes. Genotyping each individual sample can be a laborintensive and expensive process. This unit describes the technique of pooling DNA from many related affected individuals, genotyping the resulting pools, and scoring the results. This technique can greatly reduce the effort required to screen for genetic linkage and is particularly useful when applied to inbred populations. The unit includes a table showing a pool of human STRP markers developed by the Cooperative Human Linkage Center.