Unit

UNIT 2.3 Colony Hybridization to Screen for Microsatellites

  1. Thomas J. Hudson

Published Online: 1 MAY 2001

DOI: 10.1002/0471142905.hg0203s14

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Hudson, T. J. 2001. Colony Hybridization to Screen for Microsatellites. Current Protocols in Human Genetics. 14:2.3.1–2.3.5.

Author Information

  1. Whitehead Institute, Cambridge, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: AUG 1997

Abstract

Simple sequence length polymorphisms (SSLPs) for use as genetic markers are derived from the microsatellite repeat sequences found abundantly throughout eukaryotic genomes. Microsatellite DNA is identified in libraries of cloned DNA by colony hybridization to oligonucleotide probes containing simple sequence repeat (SSR) DNA, a common procedure in molecular biology laboratories. Plasmid or phagemid colonies are transferred to a nylon filter, grown briefly, fixed, and hybridized with one or more 32P-labeled oligonucleotide probes. This protocol is adapted for the rapid processing of many filters at once and optimized for identification of long stretches of uninterrupted repetitive DNA sequences.