Unit

UNIT 2.6 Genotyping by Ligation Assays

  1. Deborah A. Nickerson,
  2. Wendy Ankener,
  3. Claire Delahunty,
  4. Pui-Yan Kwok

Published Online: 1 MAY 2001

DOI: 10.1002/0471142905.hg0206s10

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Nickerson, D. A., Ankener, W., Delahunty, C. and Kwok, P.-Y. 2001. Genotyping by Ligation Assays. Current Protocols in Human Genetics. 10:2.6:2.6.1–2.6.10.

Author Information

  1. University of Washington School of Medicine, Seattle, Washington

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: AUG 1996

Abstract

This unit describes two methods of genotyping DNA sequences containing known nucleotide variations. The first protocol describes a colorimetric method for genotyping DNA samples amplified by the polymerase chain reaction (PCR) using an oligonucleotide ligation assay (OLA). The second protocol describes the ligase chain reaction (LCR), a method for simultaneously amplifying and genotyping genomic DNA samples. A Support Protocol describes the preparation of modified biotin- and digoxigenin-labeled oligonucleotide primers.