UNIT 2.6 Genotyping by Ligation Assays
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Human Genetics
How to Cite
Nickerson, D. A., Ankener, W., Delahunty, C. and Kwok, P.-Y. 2001. Genotyping by Ligation Assays. Current Protocols in Human Genetics. 10:2.6:2.6.1–2.6.10.
- Published Online: 1 MAY 2001
- Published Print: AUG 1996
This unit describes two methods of genotyping DNA sequences containing known nucleotide variations. The first protocol describes a colorimetric method for genotyping DNA samples amplified by the polymerase chain reaction (PCR) using an oligonucleotide ligation assay (OLA). The second protocol describes the ligase chain reaction (LCR), a method for simultaneously amplifying and genotyping genomic DNA samples. A Support Protocol describes the preparation of modified biotin- and digoxigenin-labeled oligonucleotide primers.