Unit

UNIT 2.9 Single-Nucleotide Polymorphism Genotyping Using Microarrays

  1. Nila Patil,
  2. Nassim Nouri,
  3. Linda McAllister,
  4. Hajime Matsukaki,
  5. Thomas Ryder

Published Online: 1 MAY 2001

DOI: 10.1002/0471142905.hg0209s27

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Patil, N., Nouri, N., McAllister, L., Matsukaki, H. and Ryder, T. 2001. Single-Nucleotide Polymorphism Genotyping Using Microarrays. Current Protocols in Human Genetics. 27:2.9:2.9.1–2.9.16.

Author Information

  1. Affymetrix, Santa Clara, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: NOV 2000

This is not the most recent version of the article. View current version (1 JUL 2013)

Abstract

This unit provides a set of protocols for high throughput SNP genotyping using oligonucleotide microarrays. The basic protocol utilizes allele-specific hybridization in which the microarray provides both the allele discrimination and read-out. In the alternate approach, the allele discrimination is accomplished in solution and then the array is used to provide the read-out of the genotyping reaction.Single nucleotide polymorphisms (SNPs) offer several advantages over other genetic markers including their high density in the.