UNIT 2.10 Genotyping Using the TaqMan Assay
Published Online: 1 JAN 2008
Copyright © 2007 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Human Genetics
How to Cite
Hui, L., DelMonte, T. and Ranade, K. 2008. Genotyping Using the TaqMan Assay. Current Protocols in Human Genetics. 56:2.10:2.10.1–2.10.8.
- Published Online: 1 JAN 2008
- Published Print: JAN 2008
The 5′-nuclease allelic discrimination assay, or TaqMan assay, is a PCR-based assay for genotyping single nucleotide polymorphisms (SNPs). The region flanking the SNP is amplified in the presence of two allele-specific fluorescent probes. The probes do not fluoresce in solution because of a quencher at the 3′ end. The presence of two probes allows the detection of both alleles in a single tube. Moreover, because probes are included in the PCR, genotypes are determined without any post-PCR processing, a feature that is unavailable with most other genotyping methods. This unit describes probe and primer design and PCR conditions. Curr. Protoc. Hum. Genet. 56:2.10.1-2.10.8. © 2008 by John Wiley & Sons, Inc.