UNIT 3.3 Construction and Assay of Radiation Hybrids
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Human Genetics
How to Cite
Richard, C. W. and Washington, S. S. 2001. Construction and Assay of Radiation Hybrids. Current Protocols in Human Genetics. 00:3.3:3.3.1–3.3.9.
- Published Online: 1 MAY 2001
- Published Print: FEB 1994
This unit describes the irradiation and fusion of cells to produce radiation hybrids and the harvesting and expansion of RH colonies. A support protocol describes amplification of human chromosome fragments by PCR and analysis of those fragments by agarose gel electrophoresis.