Unit

UNIT 3.3 Construction and Assay of Radiation Hybrids

  1. Charles W. Richard III,
  2. Sarah S. Washington

Published Online: 1 MAY 2001

DOI: 10.1002/0471142905.hg0303s00

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Richard, C. W. and Washington, S. S. 2001. Construction and Assay of Radiation Hybrids. Current Protocols in Human Genetics. 00:3.3:3.3.1–3.3.9.

Author Information

  1. University of Pittsburgh, Pittsburgh, Pennsylvania

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 1994

Abstract

This unit describes the irradiation and fusion of cells to produce radiation hybrids and the harvesting and expansion of RH colonies. A support protocol describes amplification of human chromosome fragments by PCR and analysis of those fragments by agarose gel electrophoresis.