Unit

UNIT 3.6 Conversion Technology for the Separation of Maternal and Paternal Copies of Any Autosomal Chromosome in Somatic Cell Hybrids

  1. W. Edward Highsmith Jr.,
  2. Kevin J. Meyer,
  3. Victoria M. Marley,
  4. Robert B. Jenkins

Published Online: 1 OCT 2007

DOI: 10.1002/0471142905.hg0306s55

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Highsmith, W. E., Meyer, K. J., Marley, V. M. and Jenkins, R. B. 2007. Conversion Technology for the Separation of Maternal and Paternal Copies of Any Autosomal Chromosome in Somatic Cell Hybrids. Current Protocols in Human Genetics. 55:3.6:3.6.1–3.6.38.

Author Information

  1. Division of Laboratory Genetics, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota

Publication History

  1. Published Online: 1 OCT 2007
  2. Published Print: OCT 2007

Abstract

Conversion Technology (CT) is a streamlined version of somatic cell hybrid preparation that was developed to be amenable for use as a mutation detection method and as a diagnostic tool for individual patients. It is also a powerful research tool. There are two broad categories of potential applications for CT: research applications in gene mapping and identification, and clinical applications in the detection of disease-causing mutations in individual patients. CT may emerge as the gold standard for both mutation detection and haplotyping. It will likely prove valuable in mutation detection due to its ability to detect mutations that are not amenable to detection by sequencing (e.g., deep intron or promoter mutations). It will likely prove valuable in gene identification projects because genotyping of haploid chromosomes yields unequivocal haplotypes. Curr. Protoc. Hum. Genet. 55:3.6.1-3.6.38. © 2007 by John Wiley & Sons, Inc.

Keywords:

  • haplotype;
  • mutation detection;
  • somatic cell hybrids;
  • Conversion Technology