Unit

UNIT 4.9 Multicolor Fluorescence In Situ Hybridization (FISH) Approaches for Simultaneous Analysis of the Entire Human Genome

  1. Charles Lee1,
  2. Willem Rens1,
  3. Fengtang Yang2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142905.hg0409s24

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Lee, C., Rens, W. and Yang, F. 2001. Multicolor Fluorescence In Situ Hybridization (FISH) Approaches for Simultaneous Analysis of the Entire Human Genome. Current Protocols in Human Genetics. 24:4.9:4.9.1–4.9.11.

Author Information

  1. 1

    Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts

  2. 2

    University of Cambridge, Cambridge, United Kingdom

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 2000

Abstract

The ability to generate chromosome-specific paints and employ combinatorial labeling strategies has made it possible to differentiate all 24 human chromosomes in a single metaphase spread. Such technology is particularly useful when there is a limited number of metaphase spreads for analyses and when interchromosomal rearrangements are ill-defined or very complex. There are three systems currently available for simultaneous FISH analysis of all human chromosomes: spectral karyotyping (SKY), Multiplex FISH (M-FISH), and Rx-FISH. This overview discusses each of these systems and the recent advances which have made them possible.