Unit

UNIT 4.10 Mitotic Chromosome Preparations from Mouse Cells for Karyotyping

  1. Ellen C. Akeson,
  2. Muriel T. Davisson

Published Online: 1 MAY 2001

DOI: 10.1002/0471142905.hg0410s25

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Akeson, E. C. and Davisson, M. T. 2001. Mitotic Chromosome Preparations from Mouse Cells for Karyotyping. Current Protocols in Human Genetics. 25:4.10:4.10.1–4.10.19.

Author Information

  1. The Jackson Laboratory, Bar Harbor, Maine

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAY 2000

Abstract

This unit contains protocols for the preparation of mitotic chromosomes from mouse peripheral blood, Giemsa banding of those chromosomes, and classification into a karyotype, including recognition of some common pitfalls of misidentification and information for determining aberrant chromosomes. The methods described can be used to identify visible chromosomal rearrangements and their precise cytological breakpoints in the living mouse. In conjunction with fluorescent in situ hybridization (FISH), the metaphase spreads can also be used for the linear placement of loci on a chromosome and for determining the insertion site(s) of a foreign transgene.