Unit

UNIT 4.12 Principles and Applications of PRINS in Cytogenetics

  1. Jørn Koch

Published Online: 1 SEP 2004

DOI: 10.1002/0471142905.hg0412s42

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Koch, J. 2004. Principles and Applications of PRINS in Cytogenetics. Current Protocols in Human Genetics. 42:4.12:4.12.1–4.12.13.

Author Information

  1. Laboratory of Molecular Pathology, Institute of Pathology Aarhus Kommunehospital, Aarhus, Denmark

Publication History

  1. Published Online: 1 SEP 2004
  2. Published Print: JUL 2004

Abstract

A flexible, low-cost alternative to FISH, primed in situ labeling (PRINS) has traditionally been used to detect tandemly repeated target sequences in chromosomes and nuclei. The technique is capable of discriminating among closely related DNA sequences in situ and has the advantage of using very small probes which easily penetrate to almost any target. This unit describes basic PRINS and the alternative version, dideoxy-PRINS, which can increase the sensitivity of the reaction by an order of magnitude. New material on multicolor PRINS and quantitative PRINS has been added. Protocols for detection of single-copy sequences and for application to the study of in-vivo activity of DNA-modifying enzymes are planned.

Keywords:

  • molecular cytogenetics;
  • PRINS;
  • DNA detection in situ;
  • DNA synthesis in situ;
  • sequence discrimination in situ;
  • quantitative DNA detection in situ;
  • combinatorial in situ staining