Unit

UNIT 5.10 Constructing Contigs from Large-Insert Clones

  1. Simon Foote (STS content mapping)1,
  2. Vikki Marshall (BAC/PAC)1,
  3. David J. Munroe (Alu-PCR fingerprinting)2,
  4. Julia A. Segre (repetitive-element hybridization)3

Published Online: 1 MAY 2001

DOI: 10.1002/0471142905.hg0510s15

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Foote, S., Marshall, V., Munroe, D. J. and Segre, J. A. 2001. Constructing Contigs from Large-Insert Clones. Current Protocols in Human Genetics. 15:5.10:5.10.1–5.10.35.

Author Information

  1. 1

    Walter and Eliza Hall Institute, Victoria, Australia

  2. 2

    Sequana Therapeutics, LaJolla, California

  3. 3

    Howard Hughes Medical Institute, Chicago, Illinois

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: NOV 1997

Abstract

This unit describes three approaches that are widely used to define alignments between overlapping clones bearing large-insert genomic DNA and to generate extensive contiguous overlapping sets of clones (contigs). The three approaches are sequence-tagged site (STS) content mapping, repetitive-element hybridization fingerprinting, and Alu-PCR fingerprinting. Methods for isolating the necessary BAC DNA suitable for automated fluorescent sequencing and generating new STS markers are discussed in support protocols. An alternate protocol presents repetitive-element hybridization fingerprinting to detect overlaps and build contigs with full-genomic YAC libraries.