UNIT 5.10 Constructing Contigs from Large-Insert Clones
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Human Genetics
How to Cite
Foote, S., Marshall, V., Munroe, D. J. and Segre, J. A. 2001. Constructing Contigs from Large-Insert Clones. Current Protocols in Human Genetics. 15:5.10:5.10.1–5.10.35.
- Published Online: 1 MAY 2001
- Published Print: NOV 1997
This unit describes three approaches that are widely used to define alignments between overlapping clones bearing large-insert genomic DNA and to generate extensive contiguous overlapping sets of clones (contigs). The three approaches are sequence-tagged site (STS) content mapping, repetitive-element hybridization fingerprinting, and Alu-PCR fingerprinting. Methods for isolating the necessary BAC DNA suitable for automated fluorescent sequencing and generating new STS markers are discussed in support protocols. An alternate protocol presents repetitive-element hybridization fingerprinting to detect overlaps and build contigs with full-genomic YAC libraries.