Unit

UNIT 5.17 Selective Isolation of Mammalian Genes by TAR Cloning

  1. Natalay Kouprina,
  2. Vladimir Larionov

Published Online: 1 MAY 2006

DOI: 10.1002/0471142905.hg0517s49

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Kouprina, N. and Larionov, V. 2006. Selective Isolation of Mammalian Genes by TAR Cloning. Current Protocols in Human Genetics. 49:5.17:5.17.1–5.17.19.

Author Information

  1. National Cancer Institute, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2006
  2. Published Print: APR 2006

Abstract

Transformation-associated recombination (TAR) cloning provides a unique tool for selective isolation of desired chromosome segments and full-size genes from complex genomes in the form of a circular yeast artificial chromosome (YAC) up to 250 kb in size. The method has a broad application for structural and functional genomics, long-range haplotyping, mutational analysis of gene families, characterization of chromosomal rearrangements, and evolutionary studies. This unit describes a procedure for gene isolation by TAR as well as a method for conversion of YAC-TAR isolates into a bacterial artificial chromosome (BAC) form.

Keywords:

  • transformation-associated recombination cloning (TAR);
  • yeast artificial chromosome (YAC);
  • bacterial artificial chromosome (BAC);
  • gene isolation;
  • evolution;
  • long-range haplotyping