UNIT 5.17 Selective Isolation of Mammalian Genes by TAR Cloning
Published Online: 1 MAY 2006
Copyright © 2006 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Human Genetics
How to Cite
Kouprina, N. and Larionov, V. 2006. Selective Isolation of Mammalian Genes by TAR Cloning. Current Protocols in Human Genetics. 49:5.17:5.17.1–5.17.19.
- Published Online: 1 MAY 2006
- Published Print: APR 2006
Transformation-associated recombination (TAR) cloning provides a unique tool for selective isolation of desired chromosome segments and full-size genes from complex genomes in the form of a circular yeast artificial chromosome (YAC) up to 250 kb in size. The method has a broad application for structural and functional genomics, long-range haplotyping, mutational analysis of gene families, characterization of chromosomal rearrangements, and evolutionary studies. This unit describes a procedure for gene isolation by TAR as well as a method for conversion of YAC-TAR isolates into a bacterial artificial chromosome (BAC) form.
- transformation-associated recombination cloning (TAR);
- yeast artificial chromosome (YAC);
- bacterial artificial chromosome (BAC);
- gene isolation;
- long-range haplotyping