Unit

UNIT 5.19 Large-Scale BAC Clone Restriction Digest Fingerprinting

  1. Carrie A. Mathewson,
  2. Jacqueline E. Schein,
  3. Marco A. Marra

Published Online: 1 APR 2007

DOI: 10.1002/0471142905.hg0519s53

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Mathewson, C. A., Schein, J. E. and Marra, M. A. 2007. Large-Scale BAC Clone Restriction Digest Fingerprinting. Current Protocols in Human Genetics. 53:5.19:5.19.1–5.19.21.

Author Information

  1. Canada's Michael Smith Genome Sciences Center Vancouver, British Columbia, Canada

Publication History

  1. Published Online: 1 APR 2007
  2. Published Print: APR 2007

Abstract

Restriction digest fingerprinting is a common method for characterizing large insert genomic clones, e.g., bacterial artificial chromosome (BAC), P1 artificial chromosome (PAC) and Fosmid clones. This clone fingerprinting method has been widely applied in the construction of clone-based physical maps, which have been used as positional cloning resources as well as to support directed and genome-wide sequencing efforts. This unit describes a robust, large-scale procedure for generation of agarose gel–based clone fingerprints from BAC clones.

Keywords:

  • restriction digest fingerprint;
  • bacterial artificial chromosome (BAC);
  • BAC DNA isolation;
  • agarose gel electrophoresis;
  • BAC fingerprint map