Unit

UNIT 6.1 Isolation of Exons from Cloned DNA by Exon Trapping

  1. Paul E. Nisson (internal exon trapping)1,
  2. Paul C. Watkins (internal exon trapping)2,
  3. David B. Krizman (3′ terminal exon trapping)3

Published Online: 1 MAY 2001

DOI: 10.1002/0471142905.hg0601s03

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Nisson, P. E., Watkins, P. C. and Krizman, D. B. 2001. Isolation of Exons from Cloned DNA by Exon Trapping. Current Protocols in Human Genetics. 3:6.1:6.1.1–6.1.27.

Author Information

  1. 1

    Life Technologies, Gaithersburg, Maryland

  2. 2

    Sequana Therapeutics, La Jolla, California

  3. 3

    National Center for Human Genome Research, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: NOV 1994

Abstract

Exon trapping is an RNA polymerase chain reaction (PCR) method to clone expressed sequences or exons directly from mammalian genomic DNA. The Basic Protocol 1 basic protocol in this unit describes the method for trapping internal exons from cosmid clones and the second basic protocol describes trapping of 3 terminal exons. An Alternate Protocol describes 3 terminal exon trapping, which avoids subcloning of target DNA by ligating it to the vector for direct transfection. A Support Protocol describes a rapid cloning procedure using uracil DNA glycosylase.