UNIT 6.1 Isolation of Exons from Cloned DNA by Exon Trapping
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Human Genetics
How to Cite
Nisson, P. E., Watkins, P. C. and Krizman, D. B. 2001. Isolation of Exons from Cloned DNA by Exon Trapping. Current Protocols in Human Genetics. 3:6.1:6.1.1–6.1.27.
- Published Online: 1 MAY 2001
- Published Print: NOV 1994
Exon trapping is an RNA polymerase chain reaction (PCR) method to clone expressed sequences or exons directly from mammalian genomic DNA. The Basic Protocol 1 basic protocol in this unit describes the method for trapping internal exons from cosmid clones and the second basic protocol describes trapping of 3 terminal exons. An Alternate Protocol describes 3 terminal exon trapping, which avoids subcloning of target DNA by ligating it to the vector for direct transfection. A Support Protocol describes a rapid cloning procedure using uracil DNA glycosylase.