UNIT 6.3 Direct Selection of cDNAs Using Genomic Contigs
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Human Genetics
How to Cite
Lovett, M. 2001. Direct Selection of cDNAs Using Genomic Contigs. Current Protocols in Human Genetics. 00:6.3:6.3.1–6.3.15.
- Published Online: 1 MAY 2001
- Published Print: FEB 1994
In the Basic Protocol, uncloned cDNA or cDNA from a library with highly repetitive sequences suppressed are hybridized to biotinylated genomic DNAs. The selected sequences are amplified by PCR and rehybridized to create a secondary-selected enriched cDNA library. Biotinylation of cloned genomic DNA is presented in the first support protocol. Linker addition and suppression of repeats in uncloned DNA is presented in the second support protocol and insert amplification and suppression of repeats from libraries is detailed in the third support protocol.In the Basic Protocol, uncloned cDNA or cDNA from a library with highly repetitive sequences suppressed are hybridized to biot.