UNIT 7.10 DNA Mutation Detection Using Denaturing High-Performance Liquid Chromatography (DHPLC)
Published Online: 1 FEB 2006
Copyright © 2001 John Wiley & Sons, Inc. All rights reserved.
Lab Protocol Title
Current Protocols in Human Genetics
How to Cite
Yu, B., Sawyer, N. A., Chiu, C., Oefner, P. J. and Underhill, P. A. 2006. DNA Mutation Detection Using Denaturing High-Performance Liquid Chromatography (DHPLC). Current Protocols in Human Genetics. 48:7.10:7.10.1–7.10.14.
- Published Online: 1 FEB 2006
- Published Print: JAN 2006
DHPLC is an efficient method for candidate gene scanning with a high level of automation. Single-base substitutions and insertions or deletions of up to 1.5 kb in PCR amplified DNA fragments can be detected. The method exploits the differential retention of homoduplex and heteroduplex DNA species under conditions of partial thermal denaturation. DHPLC provides a useful platform for high-throughput mutation detection and SNP discovery.
- denaturing high performance liquid chromatography;
- mutation detection;
- DNA heteroduplex;
- temperature-modulated fractionation;