Unit

UNIT 7.10 DNA Mutation Detection Using Denaturing High-Performance Liquid Chromatography (DHPLC)

  1. Bing Yu1,
  2. Nicole A. Sawyer2,
  3. Christine Chiu3,
  4. Peter J. Oefner4,
  5. Peter A. Underhill4

Published Online: 1 FEB 2006

DOI: 10.1002/0471142905.hg0710s48

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Yu, B., Sawyer, N. A., Chiu, C., Oefner, P. J. and Underhill, P. A. 2006. DNA Mutation Detection Using Denaturing High-Performance Liquid Chromatography (DHPLC). Current Protocols in Human Genetics. 48:7.10:7.10.1–7.10.14.

Author Information

  1. 1

    Department of Molecular and Clinical Genetics, Central Clinical School and SUPAMAC The University of Sydney, New South Wales, Australia

  2. 2

    SUPAMAC, The University of Sydney, New South Wales, Australia

  3. 3

    Agnes Ginges Centre for Molecular Cardiology, Centenary Institute, New South Wales, Australia

  4. 4

    Stanford University, Pasadena, California

Publication History

  1. Published Online: 1 FEB 2006
  2. Published Print: JAN 2006

Abstract

DHPLC is an efficient method for candidate gene scanning with a high level of automation. Single-base substitutions and insertions or deletions of up to 1.5 kb in PCR amplified DNA fragments can be detected. The method exploits the differential retention of homoduplex and heteroduplex DNA species under conditions of partial thermal denaturation. DHPLC provides a useful platform for high-throughput mutation detection and SNP discovery.

Keywords:

  • denaturing high performance liquid chromatography;
  • mutation detection;
  • DNA heteroduplex;
  • temperature-modulated fractionation;
  • high-throughput