UNIT 7.12 Single-Strand Conformation Polymorphism Analysis Using Capillary Electrophoresis
Published Online: 1 MAY 2003
Copyright © 2001 John Wiley & Sons, Inc. All rights reserved.
Lab Protocol Title
Current Protocols in Human Genetics
How to Cite
Larsen, L. A., Christiansen, M., Vuust, J. and Andersen, P. S. 2003. Single-Strand Conformation Polymorphism Analysis Using Capillary Electrophoresis. Current Protocols in Human Genetics. 36:7.12:7.12.1–7.12.10.
- Published Online: 1 MAY 2003
- Published Print: JAN 2003
Single-strand conformation polymorphism (SSCP) is one of the most frequently used mutation detection methods. This unit describes a method of SSCP with automated analysis by capillary electrophoresis in order to increase the capacity and throughput. A protocol is provided for sample preparation. For a medium throughput laboratory, a single capillary instrument, as described in this unit, may be quite sufficient. In many cases, however, screening for mutations in large population groups requires a high throughput, and this is best obtained through the use of a multi-capillary instrument, as discussed.