Unit

UNIT 8.4 Preparation, Culture, and Analysis of Amniotic Fluid Samples

  1. Patricia Minehart Meron

Published Online: 1 MAY 2001

DOI: 10.1002/0471142905.hg0804s05

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Meron, P. M. 2001. Preparation, Culture, and Analysis of Amniotic Fluid Samples. Current Protocols in Human Genetics. 00:8.4:8.4.1–8.4.17.

Author Information

  1. Brigham and Women's Hospital, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAY 1995

This is not the most recent version of the article. View current version (1 JUL 2012)

Abstract

The Basic Protocol describes an in situ method for the preparation, culture, harvest, and analysis of amniotic fluid samples. In this procedure, cells are grown and harvested for metaphase spreads on glass coverslips. The metaphase spreads are analyzed without being removed from the coverslips. Support Protocol 1 describes a harvesting technique that yields higher-resolution banding. Support Protocol 2 describes a method for growing more cells by passaging cells from a backup monolayer culture. An Alternate Protocol describes a flask method for the preparation, culture, harvest, and analysis of amniotic fluid samples that can be used instead of the in situ method. Accompanying Alternate Protocol is Support Protocol 3 that describes subculturing cells from flasks.