Unit

UNIT 8.4 Preparation, Culture, and Analysis of Amniotic Fluid Samples

  1. Patricia Minehart Miron

Published Online: 1 JUL 2012

DOI: 10.1002/0471142905.hg0804s74

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Minehart Miron, P. 2012. Preparation, Culture, and Analysis of Amniotic Fluid Samples. Current Protocols in Human Genetics. 74:8.4:8.4.1–8.4.14.

Author Information

  1. UMass Memorial Medical Center, Worcester, Massachusetts

Publication History

  1. Published Online: 1 JUL 2012

Abstract

Amniotic fluid obtained via amniocentesis provides a source of fetal material used in prenatal diagnosis. The fluid is used directly for biochemical analyses and as a source of fetal cells for biochemical assays, DNA testing, and chromosome studies. This unit describes an in situ method for the preparation, culture, and harvest of amniotic fluid samples for chromosome analysis. Cells are grown, harvested for metaphase spreads, and analyzed on glass coverslips. The unit also describes methods to obtain cells for additional studies (such as molecular genetic analyses) by growing cells in flasks either following passaging of cells from a glass coverslip culture or by directly establishing a flask culture from the amniotic fluid specimen. When cells are grown in flasks, they must be removed from the flask with trypsin before they can be used in studies. Curr. Protoc. Hum. Genet. 74:8.4.1-8.4.14. © 2012 by John Wiley & Sons, Inc.

Keywords:

  • cytogenetics;
  • amniotic fluid;
  • clinical;
  • metaphase chromosomes;
  • prenatal diagnosis