Unit

UNIT 9.10 Single-Cell DNA and FISH Analysis for Application to Preimplantation Genetic Diagnosis

  1. Samuel S. Chong1,
  2. Robert E. Gore-Langton1,
  3. Mark R. Hughes1,
  4. Stanislawa Weremowicz2

Published Online: 1 JAN 2010

DOI: 10.1002/0471142905.hg0910s64

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Chong, S. S., Gore-Langton, R. E., Hughes, M. R. and Weremowicz, S. 2010. Single-Cell DNA and FISH Analysis for Application to Preimplantation Genetic Diagnosis. Current Protocols in Human Genetics. 64:9.10:9.10.1–9.10.39.

Author Information

  1. 1

    Georgetown University Medical Center, Washington, D.C.

  2. 2

    Harvard Medical School and Brigham and Women's Hospital, Boston, Massachusetts

Publication History

  1. Published Online: 1 JAN 2010
  2. Published Print: JAN 2010

Abstract

Preimplantation genetic testing, which includes preimplantation genetic diagnosis (PGD) and preimplantation genetic screening (PGS), is a form of a very early prenatal testing. The goal of this method is to avoid transfer of embryos affected with a specific genetic disease or condition. This unit describes the steps involved in amplifying DNA from a single blastomere and specific assays for detecting a variety of DNA mutations. For some assays, whole-genome amplification by primer-extension preamplification (PEP) is performed prior to analysis. Support protocols describe the biopsy of one or two blastomeres from the developing preimplantation embryo, isolation for further investigation of all blastomeres from embryos shown to have the mutant allele, and isolation of single lymphocytes or lymphoblastoid cells as models for single-cell DNA analysis. A procedure for FISH analysis on single interphase blastomeres is provided along with support protocols for probe preparation and probe validation, which is recommended as a preliminary step before performing any PGD or PGS FISH analysis. Curr. Protoc. Hum. Genet. 64:9.10.1-9.10.39. © 2010 by John Wiley & Sons, Inc.

Keywords:

  • PGD;
  • PGS;
  • blastomere biopsy;
  • mutation detection;
  • FISH;
  • probe validation