UNIT 9.11 Protein Truncation Test
Published Online: 1 SEP 2004
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Human Genetics
How to Cite
Vossen, R. and den Dunnen, J. T. 2004. Protein Truncation Test. Current Protocols in Human Genetics. 42:9.11:9.11.1–9.11.23.
- Published Online: 1 SEP 2004
- Published Print: JUL 2004
The protein truncation test detects mutations at the protein level that lead to premature translation termination. The method has evolved considerably since is original publication in this manual. This thoroughly revised unit describes what is now the preferred method for performing the protein truncation test. Transcription and translation are performed in separate reactions; during translation, biotin-labeled or N-terminally tagged proteins are synthesized. The translation products are detected on immunoblots via chemiluminescence. An Alternate Protocol using coupled in vitro transcription/translation and radiolabeled proteins is also presented.