UNIT 9.17 Bone Marrow Engraftment Studies
Published Online: 1 JUL 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Human Genetics
How to Cite
Vnencak-Jones, C. L. 2009. Bone Marrow Engraftment Studies. Current Protocols in Human Genetics. 62: 9.17:9.17.1–9.17.34.
- Published Online: 1 JUL 2009
- Published Print: JUL 2009
Bone marrow engraftment studies are used to evaluate the level of donor versus recipient cells in post-transplant peripheral blood or bone marrow specimens. Unique DNA fingerprints identified from the recipient and the donor are used to determine the proportion of each contained within the total DNA extracted from the post-transplant specimen. These percentages correspond to relative amounts of donor and recipient cells in the specimen. Engraftment studies are sequentially performed on transplant patients to monitor closely the levels of donor and recipient cells so that appropriate therapeutic intervention can proceed if and when needed. This unit describes the use of fluorescent PCR for amplification of genomic short tandem repeats (STR). STR analysis is now considered the gold standard for engraftment studies and provides a quick and accurate assessment of the contribution of both donor and/or recipient hematopoietic cells in post-transplantation specimens. Curr. Protoc. Hum. Genet. 62:9.17.1-9.17.34. © 2009 by John Wiley & Sons, Inc.
- allogeneic bone marrow transplantation;
- DNA typing;
- fluorescent PCR;
- short tandem repeats