Unit

UNIT 9.18 Infectious Diseases Testing

  1. Gregory L. Blakey1,
  2. Ruth Ann Luna2,
  3. James Versalovic2

Published Online: 1 NOV 2005

DOI: 10.1002/0471142905.hg0918s47

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Blakey, G. L., Luna, R. A. and Versalovic, J. 2005. Infectious Diseases Testing. Current Protocols in Human Genetics. 47:9.18:9.18.1–9.18.24.

Author Information

  1. 1

    Baylor College of Medicine, Houston, Texas

  2. 2

    Baylor College of Medicine and Texas Children's Hospital, Houston, Texas

Publication History

  1. Published Online: 1 NOV 2005
  2. Published Print: OCT 2005

Abstract

Molecular methods have been applied widely for the diagnosis of infectious diseases. Beginning with solution hybridization in the early 1990s, multiple methods for nucleic acid hybridization and amplification have been introduced into the laboratory for the identification and characterization of microbial pathogens. This unit contains examples of several basic approaches for microbial detection or characterization in the laboratory. Methods in this chapter include automated nucleic acid extraction, direct detection of microbial pathogens, and characterization of pathogens by DNA sequencing or typing. Any of these methods could be customized for the characterization of bacteria, fungi, parasites, or viruses.

Keywords:

  • automated DNA extraction;
  • end-point PCR;
  • real-time PCR;
  • pyrosequencing;
  • real-time DNA sequencing;
  • rep-PCR