Unit

UNIT 10.2 Metaphase Harvest and Cytogenetic Analysis of Malignant Hematological Specimens

  1. Paola Dal Cin,
  2. Cynthia McLaughlin

Published Online: 1 APR 2012

DOI: 10.1002/0471142905.hg1002s73

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Cin, P. D. and McLaughlin, C. 2012. Metaphase Harvest and Cytogenetic Analysis of Malignant Hematological Specimens. Current Protocols in Human Genetics. 73:10.2:10.2.1–10.2.15.

Author Information

  1. CAMD, Cytogenetics Laboratory, Brigham and Women's Hospital, Boston, Massachusetts

Publication History

  1. Published Online: 1 APR 2012
  2. Published Print: APR 2012

Abstract

Cytogenetic analysis of malignant hematological disease is an important methodology used by clinicians and researchers, as observations of clonal chromosomal abnormalities have been shown to have both diagnostic and prognostic significance. The Basic Protocol describes culture, harvest, and preparation of chromosome spreads from bone marrow aspirates. Three alternate protocols describe adaptations of the Basic Protocol for preparation of specimens from chronic lymphocytic leukemia (CLL) blood/bone marrow, plasma cell disorders, mainly bone marrow multiple myeloma (MM), and “solid” hematological samples including lymph nodes and splenic tissue. The modifications involve slightly different culture and harvest methods that are necessary to assure optimal results. Curr. Protoc. Hum. Genet. 73:10.2.1-10.2.15. © 2012 by John Wiley & Sons, Inc.

Keywords:

  • lymph node;
  • B cell cocktail stimulation;
  • chromosome spreads;
  • mitogen;
  • bone marrow;
  • unstimulated culture