Unit

UNIT 10.4 Molecular Analysis of DNA Rearrangements in Leukemias and Non-Hodgkin's Lymphomas

  1. Janina Longtine,
  2. Edward Fox,
  3. Carol Reynolds,
  4. Jeffrey Sklar

Published Online: 1 MAY 2001

DOI: 10.1002/0471142905.hg1004s02

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Longtine, J., Fox, E., Reynolds, C. and Sklar, J. 2001. Molecular Analysis of DNA Rearrangements in Leukemias and Non-Hodgkin's Lymphomas. Current Protocols in Human Genetics. 2:10.4:10.4.1–10.4.28.

Author Information

  1. Brigham and Women's Hospital, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: AUG 1994

This is not the most recent version of the article. View current version (1 OCT 2010)

Abstract

Genetic markers for leukemias and lymphomas include chromosomal translocations and antigen-receptor gene rearrangements. Clonal rearrangements of immunoglobulin or T cell receptor (TCR) genes reflect clonal proliferations of lymphocytes, a characteristic feature of lymphoid neoplasia. These rearrangements can be detected as described in this unit by Southern blot hybridization or, in many instances, the polymerase chain reaction (PCR). Specific chromosomal translocations can also serve as markers for clonality, for malignant transformation, and for various defined subtypes of hematopoietic cancers. PCR protocols are described for detection of the two most commonly assayed translocations, t(9;22) of chronic myelogenous leukemia or acute lymphoblastic leukemia, and t(14;18) of follicular lymphomas.