UNIT 10.4 Molecular Analysis of DNA Rearrangements in Leukemias and Non-Hodgkin's Lymphomas
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Human Genetics
How to Cite
Longtine, J., Fox, E., Reynolds, C. and Sklar, J. 2001. Molecular Analysis of DNA Rearrangements in Leukemias and Non-Hodgkin's Lymphomas. Current Protocols in Human Genetics. 2:10.4:10.4.1–10.4.28.
- Published Online: 1 MAY 2001
- Published Print: AUG 1994
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Genetic markers for leukemias and lymphomas include chromosomal translocations and antigen-receptor gene rearrangements. Clonal rearrangements of immunoglobulin or T cell receptor (TCR) genes reflect clonal proliferations of lymphocytes, a characteristic feature of lymphoid neoplasia. These rearrangements can be detected as described in this unit by Southern blot hybridization or, in many instances, the polymerase chain reaction (PCR). Specific chromosomal translocations can also serve as markers for clonality, for malignant transformation, and for various defined subtypes of hematopoietic cancers. PCR protocols are described for detection of the two most commonly assayed translocations, t(9;22) of chronic myelogenous leukemia or acute lymphoblastic leukemia, and t(14;18) of follicular lymphomas.