UNIT 10.5 Molecular Analysis of Gene Amplification in Tumors
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Human Genetics
How to Cite
Wasson, J. C. and Brodeur, G. M. 2001. Molecular Analysis of Gene Amplification in Tumors. Current Protocols in Human Genetics. 2:10.5:10.5.1–10.5.18.
- Published Online: 1 MAY 2001
- Published Print: AUG 1994
Detection of gene amplification in human cancer cells has both clinical and biological importance. Amplified genes can be classified in one of two categoriesas oncogenes or as genes conferring drug resistance. Both types of gene amplification may alter clinical management of the patient. The basic protocol describes preparation and quantitation of DNA from tumor tissue and the use of conventional Southern blot hybridization analysis to detect and quantify gene amplification. The first alternate protocol provides an approach to quickly screen tumor samples for gene amplification using slot blot hybridization analysis. The second alternate protocol describes the use of the polymerase chain reaction (PCR) for analyzing tumors that may be difficult to analyze because of degradation or limited amounts of DNA. A Support Protocol explains the proper methods for obtaining, processing, storing, and shipping tumor tissue for DNA analysis.