UNIT 11.1 Genetic Analyses on DNA Microarrays
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Human Genetics
How to Cite
Stein, L. D. 2001. Genetic Analyses on DNA Microarrays. Current Protocols in Human Genetics. 24:11.1:11.1.1–11.1.8.
- Published Online: 1 MAY 2001
- Published Print: FEB 2000
A great stir has coursed through the genetics community with the advent in 1996 of “chip technologies,” a series of related techniques that allow DNA hybridization-based studies to be performed with unprecedented speed and parallelism. All chip technologies have in common a microarray, a small glass chip approximately one square centimeter in area, to which nucleotide sequences are bound. Fluorescently labeled nucleic acids are hybridized to the microarray and imaged with a laser scanner or fluorescence microscope. This unit offers an overview of the two dominant technologies, cDNA microarrays and oligonucleotide chips. It concludes with a discussion regarding how well microarrays perform real-world expression analysis.