Unit

UNIT 11.1 Genetic Analyses on DNA Microarrays

  1. Lincoln D. Stein

Published Online: 1 MAY 2001

DOI: 10.1002/0471142905.hg1101s24

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Stein, L. D. 2001. Genetic Analyses on DNA Microarrays. Current Protocols in Human Genetics. 24:11.1:11.1.1–11.1.8.

Author Information

  1. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 2000

Abstract

A great stir has coursed through the genetics community with the advent in 1996 of “chip technologies,” a series of related techniques that allow DNA hybridization-based studies to be performed with unprecedented speed and parallelism. All chip technologies have in common a microarray, a small glass chip approximately one square centimeter in area, to which nucleotide sequences are bound. Fluorescently labeled nucleic acids are hybridized to the microarray and imaged with a laser scanner or fluorescence microscope. This unit offers an overview of the two dominant technologies, cDNA microarrays and oligonucleotide chips. It concludes with a discussion regarding how well microarrays perform real-world expression analysis.