Unit

UNIT 11.3 Profiling Human Gene Expression with cDNA Microarrays

  1. Yuan Jiang,
  2. John Lueders,
  3. Arthur Glatfelter,
  4. Chris Gooden,
  5. Michael Bittner

Published Online: 1 MAY 2001

DOI: 10.1002/0471142905.hg1103s00

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Jiang, Y., Lueders, J., Glatfelter, A., Gooden, C. and Bittner, M. 2001. Profiling Human Gene Expression with cDNA Microarrays. Current Protocols in Human Genetics. 27:11.3:11.3.1–11.3.26.

Author Information

  1. National Human Genome Research Institute, NIH, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 1994

Abstract

As a result of genomics initiatives worldwide, it has become increasingly easy to obtain cDNA clones representing the 3 ends of many human genes. This unit describes methods that allow these clones to be used as hybridization detectors in a highly parallel assay of gene expression. Protocols are provided for preparing cDNA microarrays, extracting RNA from cells of interest and preparing fluorescently labeled cDNA representations of the message pools, and hybridizing the labeled cDNAs to the microarrays.