Unit

UNIT 11.5 Differential Display of mRNA by PCR

  1. F.J. Livesey1,
  2. S.P. Hunt2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142905.hg1105s10

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Livesey, F. and Hunt, S. 2001. Differential Display of mRNA by PCR. Current Protocols in Human Genetics. 30:11.5:11.5.1–11.5.11.

Author Information

  1. 1

    Harvard Medical School, Boston, Massachusetts

  2. 2

    University College London, London, United Kingdom

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: AUG 1996

Abstract

This unit outlines the polymerase chain reaction (PCR)-based technique of mRNA differential display, which identifies genes that are differentially expressed between cells or tissues. The basic protocol describes the actual differential display PCR reaction along with details of the identification, reamplification, and cloning of candidate differentially expressed genes. A support protocol provides instructions on removing contaminating genomic DNA from the RNA samples and reverse transcribing the purified RNA to produce the cDNA used in the subsequent PCR reactions.