UNIT 11.5 Differential Display of mRNA by PCR
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Human Genetics
How to Cite
Livesey, F. and Hunt, S. 2001. Differential Display of mRNA by PCR. Current Protocols in Human Genetics. 30:11.5:11.5.1–11.5.11.
- Published Online: 1 MAY 2001
- Published Print: AUG 1996
This unit outlines the polymerase chain reaction (PCR)-based technique of mRNA differential display, which identifies genes that are differentially expressed between cells or tissues. The basic protocol describes the actual differential display PCR reaction along with details of the identification, reamplification, and cloning of candidate differentially expressed genes. A support protocol provides instructions on removing contaminating genomic DNA from the RNA samples and reverse transcribing the purified RNA to produce the cDNA used in the subsequent PCR reactions.