UNIT 11.6 One-Step Enzymatic Purification of PCR Products for Direct Sequencing
Published Online: 1 NOV 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Human Genetics
How to Cite
Bum Kim, J. and Blackshaw, S. 2001. One-Step Enzymatic Purification of PCR Products for Direct Sequencing. Current Protocols in Human Genetics. 30:11.6:11.6.1–11.6.4.
- Published Online: 1 NOV 2001
- Published Print: AUG 2001
The polymerase chain reaction (PCR) has become a powerful and widespread method for analyzing DNA. This protocol offers convenient enzymatic purification of PCR products for direct sequencing without the need for further subcloning, precipitation, or column purification. All reactions may be performed in 96-well PCR plates and are compatible with large-scale sequencing. This method is designed to be convenient and rapid. It is suitable for a variety of PCR templates, including plasmid, lambda, and genomic DNA.