Unit

UNIT 11.6 One-Step Enzymatic Purification of PCR Products for Direct Sequencing

  1. Jae Bum Kim,
  2. Seth Blackshaw

Published Online: 1 NOV 2001

DOI: 10.1002/0471142905.hg1106s30

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Bum Kim, J. and Blackshaw, S. 2001. One-Step Enzymatic Purification of PCR Products for Direct Sequencing. Current Protocols in Human Genetics. 30:11.6:11.6.1–11.6.4.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 NOV 2001
  2. Published Print: AUG 2001

Abstract

The polymerase chain reaction (PCR) has become a powerful and widespread method for analyzing DNA. This protocol offers convenient enzymatic purification of PCR products for direct sequencing without the need for further subcloning, precipitation, or column purification. All reactions may be performed in 96-well PCR plates and are compatible with large-scale sequencing. This method is designed to be convenient and rapid. It is suitable for a variety of PCR templates, including plasmid, lambda, and genomic DNA.