Unit

UNIT 11.10 High-Throughput Quantitative Real-Time PCR

  1. Zoltan P. Arany

Published Online: 1 JUL 2008

DOI: 10.1002/0471142905.hg1110s58

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Arany, Z. P. 2008. High-Throughput Quantitative Real-Time PCR. Current Protocols in Human Genetics. 58:11.10:11.10.1–11.10.11.

Author Information

  1. Cardiovascular Institute, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 JUL 2008
  2. Published Print: JUL 2008

Abstract

Recent technical advances in quantitative real-time PCR (qRT-PCR) have allowed for extensive miniaturization, thereby rendering the technique amenable to high-throughput assays. Large numbers of different nucleic acids can now rapidly be measured quantitatively. Many investigations can benefit from this approach, including determination of gene expression in hundreds of samples, determination of hundreds of genes in a few samples, or even quantification of nucleic acids other than mRNA. A simple technique is described here to quantify 1880 transcripts of choice from any number of starting RNA samples. Curr. Protoc. Hum. Genet. 58:11.10.1–11.10.11. © 2008 by JohnWiley & Sons, Inc.

Keywords:

  • quantitative real-time PCR;
  • qRT-PCR;
  • high-throughput;
  • gene expression