UNIT 11.11 Digital Gene Expression by Tag Sequencing on the Illumina Genome Analyzer
Published Online: 1 APR 2010
Copyright © 2010 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Human Genetics
How to Cite
Morrissy, S., Zhao, Y., Delaney, A., Asano, J., Dhalla, N., Li, I., McDonald, H., Pandoh, P., Prabhu, A.-L., Tam, A., Hirst, M. and Marra, M. 2010. Digital Gene Expression by Tag Sequencing on the Illumina Genome Analyzer. Current Protocols in Human Genetics. 65:11.11:11.11.1–11.11.36.
- Published Online: 1 APR 2010
- Published Print: APR 2010
This unit provides a protocol for performing digital gene expression profiling on the Illumina Genome Analyzer sequencing platform. Tag sequencing (Tag-seq) is an implementation of the LongSAGE protocol on the Illumina sequencing platform that increases utility while reducing both the cost and time required to generate gene expression profiles. The ultra-high-throughput sequencing capability of the Illumina platform allows the cost-effective generation of libraries containing an average of 20 million tags, a 200-fold improvement over classical LongSAGE. Tag-seq has less sequence composition bias, leading to a better representation of AT-rich tag sequences, and allows a more accurate profiling of a subset of the transcriptome characterized by AT-rich genes expressed at levels below the threshold of detection of LongSAGE (Morrissy et al., 2009). Curr. Protoc. Hum. Genet. 65:11.11.1-11.11.36 © 2010 by John Wiley & Sons, Inc.
- gene expression;