UNIT 12.1 Biosafety in Handling Gene Transfer Vectors
Published Online: 1 AUG 2006
Copyright © 2006 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Human Genetics
How to Cite
Braun, A. 2006. Biosafety in Handling Gene Transfer Vectors. Current Protocols in Human Genetics. 50:12.1.1–12.1.18.
- Published Online: 1 AUG 2006
- Published Print: JUL 2006
This unit is devoted to safety issues that must be considered when generating and working with vectors for gene therapy. A short description of the relevant regulatory bodies and mechanisms is followed by a general discussion of containment and safety procedures. This is followed by vector-specific guidelines for nine commonly used vector systems, including adenovirus, vaccinia, HSV-1, lentivirus, and nonviral vectors. Recent tragic experiences with retroviral integration near oncogene loci demonstrate the surprise effects of successful gene therapy. The unit also outlines the problems encountered when taking a promising vector to clinical trials.
- gene transfer;