UNIT 12.4 Adenoviral Vectors

  1. Tong-Chuan He

Published Online: 1 MAY 2004

DOI: 10.1002/0471142905.hg1204s40

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

He, T.-C. 2004. Adenoviral Vectors. Current Protocols in Human Genetics. 40:12.4:12.4.1–12.4.25.

Author Information

  1. The University of Chicago Medical Center, Chicago, Illinois

Publication History

  1. Published Online: 1 MAY 2004
  2. Published Print: JAN 2004


Adenoviral vectors have been widely used as efficient gene delivery vehicles for gene therapy and vaccine development, as well as gene function studies. The recently developed AdEasy system is a simple and efficient method for rapid generation of recombinant adenoviruses. Unlike traditional adenoviral vectors, the viral backbone described here is supplied as a supercoiled plasmid rather than as a linear viral DNA, facilitating backbone amplification. The recombination step is performed in E. coli rather than in mammalian cells, taking advantage of the high efficiency of homologous recombination in bacteria. This unit includes the complete set of protocols needed to generate recombinant adenoviruses using the AdEasy system.