Unit

UNIT 12.6 Particle-Mediated Gene Delivery In Vivo and In Vitro

  1. Ning-Sun Yang,
  2. Joseph Burkholder,
  3. Dennis McCabe,
  4. Veronica Neumann,
  5. Deb Fuller

Published Online: 1 MAY 2001

DOI: 10.1002/0471142905.hg1206s12

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Yang, N.-S., Burkholder, J., McCabe, D., Neumann, V. and Fuller, D. 2001. Particle-Mediated Gene Delivery In Vivo and In Vitro. Current Protocols in Human Genetics. 12:12.6:12.6.1–12.6.14.

Author Information

  1. Auragen, Inc, Middleton, Wisconsin

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 1997

Abstract

Particle-mediated or “gene gun” technology has been developed as a nonviral method for gene transfer into various mammalian tissues. Gene delivery is achieved by physical force: a strong shock wave is generated that accelerates DNA-coated gold particles to high speeds, providing them with the momentum needed to penetrate the targeted cells. This unit describes general procedures for in vivo and in vitro DNA and RNA transfections by particle-mediated delivery. The Basic Protocol and an alternate protocol address in vivo delivery to mouse skin. In vitro delivery to cryopreserved and adherent cells is also described.