UNIT 12.6 Particle-Mediated Gene Delivery In Vivo and In Vitro
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Human Genetics
How to Cite
Yang, N.-S., Burkholder, J., McCabe, D., Neumann, V. and Fuller, D. 2001. Particle-Mediated Gene Delivery In Vivo and In Vitro. Current Protocols in Human Genetics. 12:12.6:12.6.1–12.6.14.
- Published Online: 1 MAY 2001
- Published Print: FEB 1997
Particle-mediated or “gene gun” technology has been developed as a nonviral method for gene transfer into various mammalian tissues. Gene delivery is achieved by physical force: a strong shock wave is generated that accelerates DNA-coated gold particles to high speeds, providing them with the momentum needed to penetrate the targeted cells. This unit describes general procedures for in vivo and in vitro DNA and RNA transfections by particle-mediated delivery. The Basic Protocol and an alternate protocol address in vivo delivery to mouse skin. In vitro delivery to cryopreserved and adherent cells is also described.