Unit

UNIT 12.9 Production of Recombinant Adeno-Associated Viral Vectors

  1. Vivian W. Choi,
  2. Aravind Asokan,
  3. Rebecca A. Haberman,
  4. Richard Jude Samulski

Published Online: 1 APR 2007

DOI: 10.1002/0471142905.hg1209s53

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Choi, V. W., Asokan, A., Haberman, R. A. and Samulski, R. J. 2007. Production of Recombinant Adeno-Associated Viral Vectors. Current Protocols in Human Genetics. 53:12.9:12.9.1–12.9.21.

Author Information

  1. University of North Carolina, Chapel Hill, North Carolina

Publication History

  1. Published Online: 1 APR 2007
  2. Published Print: APR 2007

Abstract

Adeno-associated virus is a nonpathogenic human virus that has been developed into a gene-delivery vector due to its high efficiency of infection for many different cell types and its ability to persist and lead to long-term gene expression. This unit describes efficient methods to generate high-titer, research-grade, adenovirus-free recombinant single-stranded and self-complementary adeno-associated virus in various serotypes, along with methods to quantify the viral vectors.

Keywords:

  • Adeno-associated virus;
  • AAV;
  • gene therapy;
  • gene delivery