Unit

UNIT 12.10 Production and Titration of Lentiviral Vectors

  1. Patrick Salmon1,
  2. Didier Trono2

Published Online: 1 JUL 2007

DOI: 10.1002/0471142905.hg1210s54

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Salmon, P. and Trono, D. 2007. Production and Titration of Lentiviral Vectors. Current Protocols in Human Genetics. 54:12.10:12.10.1–12.10.24.

Author Information

  1. 1

    Department of Neuroscience, Faculty of Medicine, University of Geneva, Geneva, Switzerland

  2. 2

    School of Life Sciences, École Polytechnique Fédérale de, Lausanne and “Frontiers in Genetics,” National Center for Competence in Research, Lausanne, Switzerland

Publication History

  1. Published Online: 1 JUL 2007
  2. Published Print: JUL 2007

Abstract

Lentiviral vectors have emerged over the last decade as powerful, reliable and safe tools for stable gene transfer in a wide variety of mammalian cells. Unlike other vectors derived from oncoretroviruses, they allow for stable gene delivery into most nondividing primary cells. This is why LVs are becoming useful and promising tools for future gene and cell therapy approaches. Lentivectors (LVs) derived from HIV-1 have gradually evolved to display many desirable features aimed at increasing both their safety and their versatility. These latest designs are reviewed in this unit. This unit also describes protocols for production and titration of LVs that can be implemented in a research laboratory setting, with an emphasis on standardization to improve transposability of results between laboratories Curr. Protoc. Hum. Genet. 54:12.10.1-12.10.24. © 2007 by John Wiley & Sons, Inc.

Keywords:

  • Lentiviral vectors;
  • gene therapy;
  • gene delivery