Unit

UNIT 12.14 Oncolytic Adenoviruses: Design, Generation, and Experimental Procedures

  1. Julia Davydova,
  2. Masato Yamamoto

Published Online: 1 JUL 2013

DOI: 10.1002/0471142905.hg1214s78

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Davydova, J. and Yamamoto, M. 2013. Oncolytic Adenoviruses: Design, Generation, and Experimental Procedures. Current Protocols in Human Genetics. 78:12.14:12.14.1–12.14.21.

Author Information

  1. Division of Basic and Translational Research, Department of Surgery, University of Minnesota, Minneapolis, Minnesota

Publication History

  1. Published Online: 1 JUL 2013

Abstract

Oncolytic adenoviruses are designed to take advantage of the virus' native ability to replicate in cancer cells to induce oncolysis. Subsequently, the released viral progeny spread and kill the neighboring cancer cells. These characteristics, together with the ability of adenovirus to infect a broad spectrum of cells, its well understood replication machinery, and relative ease of manufacture have led to the intensive use of adenovirus as an anticancer agent. This unit describes cloning strategies, procedures to turn the intended design into virus, and quality analyses of resultant adenoviral vectors. Most of these procedures were optimized especially for oncolytic adenoviral vectors. Curr. Protoc. Hum. Genet. 78:12.14.1-12.14.21. © 2013 by John Wiley & Sons, Inc.

Keywords:

  • adenovirus;
  • oncolytic;
  • production;
  • design;
  • cloning;
  • strategy;
  • amplification;
  • purification