UNIT 13.2 DNA Vaccination

  1. Jeffrey B. Ulmer,
  2. Rino Rappuoli

Published Online: 1 AUG 2003

DOI: 10.1002/0471142905.hg1302s37

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Ulmer, J. B. and Rappuoli, R. 2003. DNA Vaccination. Current Protocols in Human Genetics. 37:13.2:13.2.1–13.2.9.

Author Information

  1. Chiron Corporation, Emeryville, California

Publication History

  1. Published Online: 1 AUG 2003
  2. Published Print: APR 2003


This unit details some of the key methods for setting up and testing DNA vaccines in animal models. The basic procedures are discussed, as well as alternative methods that have been developed over the past several years. The Basic Protocol gives step-by-step instructions for administering the DNA vaccine via intramuscular injection of the quadriceps muscle, while an alternate procedure details injection of the anterior tibialis.