UNIT 13.4 Gene Delivery to Muscle
Published Online: 1 FEB 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Human Genetics
How to Cite
Springer, M. L., Rando, T. A. and Blau, H. M. 2002. Gene Delivery to Muscle. Current Protocols in Human Genetics. 31:13.4:13.4.1–13.4.19.
- Published Online: 1 FEB 2002
- Published Print: OCT 2001
The delivery of genes to skeletal muscle by myoblast implantation, DNA injection, or viral transduction has therapeutic applications for human neuromuscular and systemic disorders, many of which are now represented by transgenic or “knockout” mouse models. This unit describes the isolation and retroviral transduction of mouse myoblasts, the injection of myoblasts and plasmid DNA into mouse muscle, and histological methods for analyzing the recipient muscle. A procedure describing the injection of plasmid DNA into muscle with or without electric charge is also included.