UNIT 13.4 Gene Delivery to Muscle

  1. Matthew L. Springer,
  2. Thomas A. Rando,
  3. Helen M. Blau

Published Online: 1 FEB 2002

DOI: 10.1002/0471142905.hg1304s31

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Springer, M. L., Rando, T. A. and Blau, H. M. 2002. Gene Delivery to Muscle. Current Protocols in Human Genetics. 31:13.4:13.4.1–13.4.19.

Author Information

  1. Stanford University School of Medicine, Stanford, California

Publication History

  1. Published Online: 1 FEB 2002
  2. Published Print: OCT 2001


The delivery of genes to skeletal muscle by myoblast implantation, DNA injection, or viral transduction has therapeutic applications for human neuromuscular and systemic disorders, many of which are now represented by transgenic or “knockout” mouse models. This unit describes the isolation and retroviral transduction of mouse myoblasts, the injection of myoblasts and plasmid DNA into mouse muscle, and histological methods for analyzing the recipient muscle. A procedure describing the injection of plasmid DNA into muscle with or without electric charge is also included.