Unit

UNIT 13.8 Cancer Vaccines

  1. Tim F. Greten,
  2. Elizabeth M. Jaffee

Published Online: 1 MAY 2001

DOI: 10.1002/0471142905.hg1308s14

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Greten, T. F. and Jaffee, E. M. 2001. Cancer Vaccines. Current Protocols in Human Genetics. 14:13.8:13.8.1–13.8.19.

Author Information

  1. Johns Hopkins University School of Medicine, Baltimore, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: AUG 1997

Abstract

This unit describes the use of retroviral vectors that can be successfully employed for gene transfer into both primary tumor cultures and established cell lines. The unit includes procedures for assaying the stability of the vaccine following gene transfer. Techniques for maintaining the retroviral producer lines and titering the retroviral vectors are also described. A protocol for frozen storage of the vaccine (transduced tumor cells) is provided. In addition, methods are described for characterizing the vaccine cells following gene transfer. Directions for testing the expression of the transferred gene in the transfected tumor line are also given. The final protocol provides suggestions for designing in vivo animal experiments and discusses what has to be observed in a clinical setting.