UNIT 13.9 Gene Delivery to the Airway

  1. Dongsheng Duan,
  2. Yulong Zhang,
  3. John F. Engelhardt

Published Online: 1 MAY 2001

DOI: 10.1002/0471142905.hg1309s23

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Duan, D., Zhang, Y. and Engelhardt, J. F. 2001. Gene Delivery to the Airway. Current Protocols in Human Genetics. 23:13.9:13.9.1–13.9.34.

Author Information

  1. University of Iowa, Iowa City, Iowa

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: NOV 1999

This is not the most recent version of the article. View current version (1 JUL 2013)


This unit describes generation of and gene transfer to several commonly used airway models. Isolation and transduction of primary airway epithelial cells are first described. Next, the preparation of polarized airway epithelial monolayers is outlined. Transduction of these polarized cells is also described. Methods are presented for generation of human bronchial xenografts as well as both ex vivo and in vivo gene transfer to these xenografts. Finally, a method for in vivo gene delivery to the lungs of rodents is included. Methods for evaluating transgene expression are given in the support protocols.