Unit

UNIT 13.9 Gene Delivery to the Airway

  1. Nicholas W. Keiser,
  2. John F. Engelhardt

Published Online: 1 JUL 2013

DOI: 10.1002/0471142905.hg1309s78

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Keiser, N. W. and Engelhardt, J. F. 2013. Gene Delivery to the Airway. Current Protocols in Human Genetics. 78:13.9:13.9.1–13.9.41.

Author Information

  1. University of Iowa, Iowa City, Iowa

Publication History

  1. Published Online: 1 JUL 2013

Abstract

This unit describes generation of and gene transfer to several commonly used airway models. Isolation and transduction of primary airway epithelial cells are first described. Next, the preparation of polarized airway epithelial monolayers is outlined. Transduction of these polarized cells is also described. Methods are presented for generation of tracheal xenografts, as well as both ex vivo and in vivo gene transfer to these xenografts. Finally, a method for in vivo gene delivery to the lungs of rodents is included. Methods for evaluating transgene expression are given in the support protocols. Curr. Protoc. Hum. Genet. 78:13.9.1-13.9.41. © 2013 by John Wiley & Sons, Inc.

Keywords:

  • genetics and genomics;
  • gene therapy