Unit

UNIT 15.3 Use of Zebrafish Models for the Analysis of Human Disease

  1. Hao Zhu1,
  2. Leonard I. Zon1,2

Published Online: 1 NOV 2002

DOI: 10.1002/0471142905.hg1503s34

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Zhu, H. and Zon, L. I. 2002. Use of Zebrafish Models for the Analysis of Human Disease. Current Protocols in Human Genetics. 34:15.3:15.3.1–15.3.9.

Author Information

  1. 1

    Howard Hughes Medical Institute, Chevy Chase, Maryland

  2. 2

    Children's Hospital and Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 NOV 2002
  2. Published Print: JUL 2002

Abstract

The zebrafish has emerged as a powerful animal model for human diseases. While it has long informed us about the biology of early development, it has recently come into favor for the investigation of clinically relevant problems. Genes conserved from fish to humans can be rapidly analyzed using the zebrafish embryo in what is essentially a transparent in vivo assay. This unit describes methodologies including genetic screening, targeted knockdowns, ectopic overexpression, and transgenesis.