UNIT 15.3 Use of Zebrafish Models for the Analysis of Human Disease
Published Online: 1 NOV 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Human Genetics
How to Cite
Zhu, H. and Zon, L. I. 2002. Use of Zebrafish Models for the Analysis of Human Disease. Current Protocols in Human Genetics. 34:15.3.1–15.3.9.
- Published Online: 1 NOV 2002
- Published Print: JUL 2002
The zebrafish has emerged as a powerful animal model for human diseases. While it has long informed us about the biology of early development, it has recently come into favor for the investigation of clinically relevant problems. Genes conserved from fish to humans can be rapidly analyzed using the zebrafish embryo in what is essentially a transparent in vivo assay. This unit describes methodologies including genetic screening, targeted knockdowns, ectopic overexpression, and transgenesis.