UNIT 15.4 ENU Mutagenesis in the Mouse
Published Online: 1 FEB 2004
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Human Genetics
How to Cite
McDonald, J. D. and Beier, D. 2004. ENU Mutagenesis in the Mouse. Current Protocols in Human Genetics. 39:15.4:15.4.1–15.4.8.
- Published Online: 1 FEB 2004
- Published Print: OCT 2003
This is not the most recent version of the article. View current version (14 JUL 2014)
This unit describes the treatment of laboratory mice with the mutagen N-ethyl-N-nitrosourea (ENU) to achieve very highly induced rates of mutation throughout the genome. Further, it describes several popular mating schemes designed to produce animals displaying phenotypes associated with the induced mutations.
- alkylating agent;
- spermatogonial stem cells;
- per locus mutation frequency;
- driven mutagenesis