UNIT 15.4 ENU Mutagenesis in the Mouse

  1. J. David McDonald1,
  2. David Beier2

Published Online: 1 FEB 2004

DOI: 10.1002/0471142905.hg1504s39

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

McDonald, J. D. and Beier, D. 2004. ENU Mutagenesis in the Mouse. Current Protocols in Human Genetics. 39:15.4:15.4.1–15.4.8.

Author Information

  1. 1

    Wichita State University, Wichita, Kansas

  2. 2

    Brigham and Women's Hospital, Boston, Massachusetts

Publication History

  1. Published Online: 1 FEB 2004
  2. Published Print: OCT 2003

This is not the most recent version of the article. View current version (14 JUL 2014)


This unit describes the treatment of laboratory mice with the mutagen N-ethyl-N-nitrosourea (ENU) to achieve very highly induced rates of mutation throughout the genome. Further, it describes several popular mating schemes designed to produce animals displaying phenotypes associated with the induced mutations.


  • alkylating agent;
  • spermatogonial stem cells;
  • per locus mutation frequency;
  • phenotype-;
  • driven mutagenesis