Unit

UNIT 17.2 Chromatographic Analysis of Amino and Organic Acids in Physiological Fluids to Detect Inborn Errors of Metabolism

  1. Michael Woontner,
  2. Stephen I. Goodman

Published Online: 1 NOV 2006

DOI: 10.1002/0471142905.hg1702s51

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Woontner, M. and Goodman, S. I. 2006. Chromatographic Analysis of Amino and Organic Acids in Physiological Fluids to Detect Inborn Errors of Metabolism. Current Protocols in Human Genetics. 51:17.2:17.2.1–17.2.19.

Author Information

  1. University of Colorado Health Sciences Center, Aurora, Colorado

Publication History

  1. Published Online: 1 NOV 2006
  2. Published Print: OCT 2006

Abstract

This unit describes methods for the preparation of samples for analysis of physiological amino acids and organic acids. Amino acids are analyzed by ion-exchange chromatography using an automated system. Organic acids are analyzed by gas-chromatography/mass spectrometry (GC-MS). Analysis of amino and organic acids is necessary to detect and monitor the treatment of many inborn errors of metabolism.

Keywords:

  • amino acids;
  • organic acids;
  • LC;
  • GC-MS