Unit

UNIT 18.2 Improved Protocols for the Illumina Genome Analyzer Sequencing System

  1. Michael A. Quail,
  2. Harold Swerdlow,
  3. Daniel J. Turner

Published Online: 1 JUL 2009

DOI: 10.1002/0471142905.hg1802s62

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Quail, M. A., Swerdlow, H. and Turner, D. J. 2009. Improved Protocols for the Illumina Genome Analyzer Sequencing System. Current Protocols in Human Genetics. 62:18.2:18.2.1–18.2.27.

Author Information

  1. Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, United Kingdom

Publication History

  1. Published Online: 1 JUL 2009
  2. Published Print: JUL 2009

This is not the most recent version of the article. View current version (21 JAN 2014)

Abstract

In this unit, we describe a set of improvements we have made to the standard Illumina Genome Analyzer protocols to make the sequencing process more reliable in a high-throughput environment, reduce amplification bias, narrow the distribution of insert sizes, and reliably obtain high yields of data. Curr. Protoc. Hum. Genet. 62:18.2.1-18.2.27. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • Illumina;
  • Next-Generation;
  • sequencer;
  • protocols;
  • Genome Analyzer