Unit

UNIT 19.3 Evaluation of the Mitochondrial Respiratory Chain and Oxidative Phosphorylation System Using Polarography and Spectrophotometric Enzyme Assays

  1. Antoni Barrientos,
  2. Flavia Fontanesi,
  3. Francisca Díaz

Published Online: 1 OCT 2009

DOI: 10.1002/0471142905.hg1903s63

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Barrientos, A., Fontanesi, F. and Díaz, F. 2009. Evaluation of the Mitochondrial Respiratory Chain and Oxidative Phosphorylation System Using Polarography and Spectrophotometric Enzyme Assays. Current Protocols in Human Genetics. 63:19.3:19.3.1–19.3.14.

Author Information

  1. University of Miami Miller School of Medicine, Miami, Florida

Publication History

  1. Published Online: 1 OCT 2009
  2. Published Print: OCT 2009

Abstract

The oxidative phosphorylation (OXPHOS) system consists of five multimeric complexes embedded in the mitochondrial inner membrane. They work in concert to drive the aerobic synthesis of ATP. Mitochondrial and nuclear DNA mutations affecting the accumulation and function of these enzymes are the most common cause of mitochondrial diseases and have also been associated with neurodegeneration and aging. For this reason, several approaches for the assessment of the OXPHOS system enzymes have been developed. Based on the methods described elsewhere, the assays describe methods that form a biochemical characterization of the OXPHOS system in cells and mitochondria isolated from cultured cells or tissues. Curr. Protoc. Hum. Genet. 63:19.3.1-19.3.14. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • electron transport chain;
  • mitochondria;
  • OXPHOS;
  • polarography;
  • spectrophotometry