Unit

UNIT 19.4 Evaluation of the Mitochondrial Respiratory Chain and Oxidative Phosphorylation System Using Blue Native Gel Electrophoresis

  1. Francisca Díaz,
  2. Antoni Barrientos,
  3. Flavia Fontanesi

Published Online: 1 OCT 2009

DOI: 10.1002/0471142905.hg1904s63

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Díaz, F., Barrientos, A. and Fontanesi, F. 2009. Evaluation of the Mitochondrial Respiratory Chain and Oxidative Phosphorylation System Using Blue Native Gel Electrophoresis. Current Protocols in Human Genetics. 63:19.4:19.4.1–19.4.12.

Author Information

  1. University of Miami Miller School of Medicine, Miami, Florida

Publication History

  1. Published Online: 1 OCT 2009
  2. Published Print: OCT 2009

Abstract

The oxidative phosphorylation (OXPHOS) system consists of five multimeric complexes embedded in the mitochondrial inner membrane. They work in concert to drive the aerobic synthesis of ATP. Mitochondrial and nuclear DNA mutations affecting the accumulation and function of these enzymes are the most common cause of mitochondrial diseases and have also been associated with neurodegeneration and aging. For this reason, several approaches for the assessment of the OXPHOS system enzymes have been progressively developed. Based on methods described elsewhere, the use of blue native gel electrophoresis (BNGE) techniques to routinely assess the OXPHOS system and screen for enzymatic defects in homogenates or mitochondrial preparations from tissues or cultured cells is described here. Curr. Protoc. Hum. Genet. 63:19.4.1-19.4.12. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • blue native gel electrophoresis (BNGE);
  • electron transport chain;
  • mitochondria;
  • OXPHOS