Unit

UNIT 19.5 Evaluation of the Mitochondrial Respiratory Chain and Oxidative Phosphorylation System Using Yeast Models of OXPHOS Deficiencies

  1. Flavia Fontanesi,
  2. Francisca Diaz,
  3. Antoni Barrientos

Published Online: 1 OCT 2009

DOI: 10.1002/0471142905.hg1905s63

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Fontanesi, F., Diaz, F. and Barrientos, A. 2009. Evaluation of the Mitochondrial Respiratory Chain and Oxidative Phosphorylation System Using Yeast Models of OXPHOS Deficiencies. Current Protocols in Human Genetics. 63:19.5:19.5.1–19.5.20.

Author Information

  1. University of Miami Miller School of Medicine, Miami, Florida

Publication History

  1. Published Online: 1 OCT 2009
  2. Published Print: OCT 2009

Abstract

The oxidative phosphorylation (OXPHOS) system consists of five multimeric complexes embedded in the mitochondrial inner membrane. They work in concert to drive the aerobic synthesis of ATP. Mitochondrial and nuclear DNA mutations affecting the accumulation and function of these enzymes are the most common cause of mitochondrial diseases and have also been associated with neurodegeneration and aging. Several approaches for the assessment of the OXPHOS system enzymes have been developed. Based on the methods described elsewhere, this unit describes the creation and study of yeast models of mitochondrial OXPHOS deficiencies. Curr. Protoc. Hum. Genet. 63:19.5.1-19.5.20. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • electron transport chain;
  • mitochondria;
  • OXPHOS deficiencies;
  • yeast model